ARIA

FACS ARIA-II

The FACS ARIA-II is our sorting instrument. The instrument has a very similar configuration to the LSR-II instrument to provide the opportunity to analyse celles and afterwards sort the same populations. The instrument can only be used together with the facility's operator.

Specification

Lasers

  • Coherent Sapphire 488 nm, air cooled laser (13-20 mW)
  • 635 nm JDS Uniphase HeNe, air cooled laser (10-20 mW)
  • 407 nm Point Source Violet solid state laser (10-17 mW)

Optical system

  • Detection of 14 FL-parameters
  • Detection of forward scatter light (FSC) with photo diode
  • Direction of side scatter light (SSC) and fluorescence signal towards PMTs

Blue laser detection (488 nm)*

  • 502 LP, 530/30 nm FITC
  • 556 LP, 585/42 nm PE
  • 610 LP, 616/23 nm PE-Texas Red
  • 655 LP, 695/40 nm PE-Cy5.5
  • 735 LP, 780/60 nm PE-Cy7

Red laser detection (640 nm)*

  • 660/20 nm APC
  • 690 LP, 730/45 nm Alexa Flour 700
  • 735 LP, 780/60 nm APC-Cy7

Violet laser detection (405 nm)*

  • 431/28 nm BV421
  • 502 LP, 525/50 nm BV480 and BV510
  • 595 LP, 610/20 nm BV605
  • 630 LP, 660/20 nm BV650
  • 690 LP, 720/30 nm BV711
  • 750 LP, 780/60 nm BV786

We do have additional bandpass filters that, on request, can be used in the instrument. 
Check the reference guide including a guide to setting up the color combination or use the more comprehensive list of available fluorochromes.

* Each color is just an example, you should ensure that you are using the right colors by checking the filter setting.

Sort Collection Devices

  • Two-way sorting into the following sample tube collection devices: microtubes, 12x75 mm, and 15mL
  • Four-way sorting into the following sample tube collection devices: microtubes, 12x75 mm
  • Automatic Cell Deposition Unit (ACDU) option allows for slide and plate sorting into 6, 24, 48, 96, and 384 plates

Sample Input Sizes

  • Microtubes, 12x75 mm, and 15mL

Temperature Control

  • Sample input, software-adjustable: 4, 20, 37 and 42 degrees C
  • Sample output for sort collection: water recirculation unit with variably settings